A simple, sensitive, rapid, accurate and precise derivative spectrophotometric method has been developed for the estimation of Cefixime Trihydrate in bulk and pharmaceutical dosage forms. Cefixime Trihydrate shows maximum absorbance at 288 nm. Spectrophotometrically, Cefixime Trihydrate was determined by measuring the 2D-values at 288 nm with phosphate buffer (pH-6.5) as a solvent. Beer’s law was obeyed in the concentration range of 4-18 μg/ml with coefficient of correlation 0.999. The developed method was applied directly to the analysis of the tablet preparations. R.S.D was found to be 0.0015. The result 100.16-100.54% for recovery studies by derivative method indicate that proposed method is accurate and precise for the determination of Cefixime Trihydrate in tablets. The method was completely validated and proven to be rugged. The excipients did not interfere in the analysis. The results showed that this method can be used for rapid determination of Cefixime Trihydrate in pharmaceutical tablet with precision, accuracy and specificity.
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